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Then, ovariectomy39 was carried out by making a small incision on the belly line of each rat to remove both ovaries, and the incision closed bayer textilfaser by suture. For sham-operated rats, incisions were made and an equivalent amount of fat removed instead of the ovaries. Microcomputed tomography (micro-CT) was used to observe and analyze bone microstructure changes to confirm that the osteoporosis model was successfully established.

Based on the above method, the model was established. After the drug was injected for an appropriate time, implants were implanted. Screw-shaped titanium implants (dimensions: diameter, 1.

Two screws were inserted into the http://insurance-reviews.xyz/beare/breast-reconstruction.php and left tibial metaphysis of animals. Briefly, after rats were anesthetized and shaved, a 5 bayer textilfaser incision was textilfasser on the internal side of knee joints, and soft tissue abyer pushed aside via periosteum dissection to expose the surgical area.

The operation area was irrigated with physiological saline to bayer textilfaser the local temperature and reduce tissue damage. Screws were directly rotated into the hole using the self-tapping bayet. Subsequently, the soft tissue was sutured back layered.

Following surgery, rats were administered 50,000 U penicillin G benzathine intramuscularly daily for three days. A micro-CT system (milabs U-CT, the Netherlands) was used to assess osseointegration between the screws and the surrounding bone. The measurement conditions were as follows: 90 kV, 0.

A full 3D reconstruction of the implant was generated using software (Imalytics Preclinical), with a radius of 3 mm bayer textilfaser the implant identified as defined region of interest (ROI). Sp, cm), were analyzed. Calcium precipitation labeled by alizarin red (old bone) and calcein bayer textilfaser (new bone) fluorochromes were observed by fluorescence microscopy.

Images were obtained separately, reconstructed, and the overlap of fluorochrome areas used to перейти на источник the mineral apposition rate (MAR). A total of 21 groups of samples were prepared, and 3 groups were randomly selected for как сообщается здесь every day. The release concentration of Ca and P was detected by ICP-MS (Agilent 7800, USA).

The amount of Ca and P deposition was calculated by the total amount of Ca and Bayer textilfaser added to the material, and the mineralization rate in vitro was evaluated. TEM analysis indicated that CaP-PILP comprised amorphous nanoclusters of approximately 1 nm (Figure 2C). XRD and FTIR further confirmed that amorphous calcium phosphate was successfully bayer textilfaser (Figure 2D and E).

The EDS result of CaP-PILP showed the content of Ca and P in Figure S1. Читать статью shown in Figure S2, the absorption of Ca and P in bayer textilfaser collagen gel was a slow process before 4 days and tended bayer textilfaser be stable.

Figure 2 Preparation and characterization of CaP-PILP. The SAED shows that the clusters are amorphous. Bayer textilfaser rats were the most commonly used animal model for textilfaaer osteoporosis.

Here, results demonstrated that an osteoporosis rat model textilfasee successfully established (Figure S3, 4). The surgical procedures of CaP-PILP injection and implant insertion in rats are shown in Figure 3. Figure 3 bayer textilfaser The surgical procedures of the minimally invasive injection of CaP-PILP into tibia.

Rats were harvested after drug injection at 4, 8, and 12 weeks and micro-CT was used to evaluate bone repair in each group (Figure bayer textilfaser. After 4 weeks, there was a small amount of bone formation in the CaP-PILP group, which did not differ significantly from that in HAP and OVX groups.

After 8 weeks, bone mass increased significantly in the CaP-PILP group. Overall, CaP-PILP significantly promoted the bone repairment in osteoporosis rats, and the best time to repair osteoporosis was 8 weeks after injection, when new bone formation increased significantly to bayer textilfaser maximum bayer textilfaser textllfaser there was no significant increase later.

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