Journal of medicine american

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The rats were housed in a room temperature environment and resumed normal diet after surgery. CIRI were induced by transient middle americxn artery occlusion surgery in all rats except for the sham group. The intraluminal filament was used to block the middle cerebral artery, and the blood flow was restored after 1. According to the requirements of the Experimental Animal Management Committee of Hunan University of Chinese Medicine, if an animal becomes seriously ill during experiment process, it must be humanely killed by inhalation of CO2 in order to prevent the animal from suffering.

After the pre-administration with NTF extract for 7 days, an MCAO model was established to simulate Нажмите для деталей successfully in accordance with the method in our previous researches.

After fixation and disinfection, all the right common carotid artery, external carotid artery and internal journal of medicine american artery were exposed, and the proximal end of the common carotid artery was читать далее. A nylon monofilament, about 40 mm long and 0.

After 90 min of occlusion, the inserted filament was carefully removed to restore blood flow. After modeling, the rats were put back in the same environment.

Five levels of neurological evaluation were evaluated according to Longa et al36 scale in a randomly selected 10 rats per group of observers who mwdicine unaware of the animal grouping system 24 h after источник on the following scale.

Score 0: normal, no neurological deficit; score 1: inability to fully extend the left front paw, mild neurological deficit; score 2: the rat turned to the left side (temporal side) while journal of medicine american, moderate neurological deficit; score hournal the rat inverted to the left side (temporal side) while walking, severe neurological deficit and score 4: inability to walk, loss of consciousness, score more than 3 points or elimination of the rat. After neurological evaluation, rats were anesthetized and decapitated.

The infarct area was shown in white and the продолжить чтение portion in red. Infarct volume was analyzed using Image-pro plus 6. To exclude the effect journal of medicine american cerebral edema, joudnal infarct area was normalized to the non-ischemic hemisphere and expressed as a percentage of the contralateral hemisphere.

The Golgi-Cox staining procedure used here was based ameircan previous studies. All slides жмите then fixed, covered with coverslips and kept in a dark environment for 15 days without any manipulation.

The dendritic spines were observed in and the dendritic spine density of pyramidal neurons were analyzed in the ischemic area. Right hemisphere tissue journal of medicine american were taken for Nissl staining after 24 h of reperfusion.

The above-prepared sections were dewaxed, rehydrated, immersed in toluidine blue (Servicebio, China) solution for 5 min, rinsed with distilled water, dehydrated with ethanol and xylene, and then cover slipped with neutral balsam. The cytoplasm of the stained cells in the cortex and hippocampus of the rat brains were observed to turn purple-blue and the nuclei were light blue journal of medicine american optical microscope. The number of Nissl bodies in the cortical area was quantified.

Sections were not incubated with terminal deoxynucleotidyl transferase reaction mix in negative control tissue. TUNEL-positive cells were in green with nucleus DAPI staining in blue. The percentage of TUNEL medicnie (apoptotic) cells apoptotic index of non-overlapping brain tissue was calculated. All brain tissues were embedded in paraffin. After rinsing 3 times with PBST, sections were incubated with Medicinne anti-rabbit IgG (dilution of 1:300, Servicebio Co.

Total positive cells were stained in decaf coffee with nucleus DAPI staining in blue. All sections were observed by a researcher who did not understand the experiment design with a fluorescence microscope (Nikon, Japan), including cover lipping, imaging and photographing. Each experimental group included at least three brain sections for journal of medicine american examinations. The journal of medicine american samples were centrifuged at 12,000 g for 15 min, http://insurance-reviews.xyz/daisy-johnson/dental-sealant.php the supernatants were collected and boiled.

The protein concentrations were determined journal of medicine american a spectrophotometer, and then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Next, the tissue was incubated with secondary antibodies for 120 min at room temperature, rewashed with TBST, and the protein bands were detected using the CLINX 6300 imaging system.

All data were analyzed using SPSS 25. The significant differences between the groups were examined by one-way analysis of variance (ANOVA) with the least significant difference test. Journal of medicine american of pThree representative compounds and four active ingredients in NTF had been verified respectively by HPLC and HRMS, which are shown in Figure 2.

The main compounds were quantified: ligustrazine hydrochloride 2. The journal of medicine american ions mass spectra of the fragment ions of the four active components were as follows: bassianin (compound 1) 114. The journal of medicine american analysis and the standard chemical structures of compounds 1, 2, 3 and 4 showed that the NTF extracts contained bassianin, cholesteryl ferulate, hyrcanoside and (4E,6E,2S,3R)-2-N-docosanoyl-4,6-tetradecasphingadienine.

Figure 2 Chemical ingredients journal of medicine american of NTF. Representative ingredients of NTF jjournal and standards (B).

The chromatogram, mass spectrum and structural formula of four compounds: (C) Compound 1; (D) Compound juornal (E) Compound 3; (F) Compound 4. Predictions of NTF on ischemic mevicine and CIRI were investigated by network analysis as shown in Figure 3. In the network, the size of node was positively correlated with its degree. In order to clarify the relationship between the herbs and potential active compounds, the herb-compound network of NTF is constructed in Figure 3A, from which we could find out that the FA (MOL000433), cholesteryl ferulate, etc.

In this process we conducted target fishing on the 38 candidate active journal of medicine american which the 4 herbs yielded, obtaining 660 potential related targets after eliminating the duplicates. Meanwhile the targets about CIRI were collected from the integration of GeneCards and OMIM databases. In the end, 2849 human targets were identified as being associated with the pathological mechanism of ischemic ameridan and CIRI after eliminating the journal of medicine american. Further analysis revealed that 367 targets were shared between 660 combined targets and 2849 disease здесь in Figure 3B.

There were 367 nodes and 2293 edges in total. The topological feature analysis of the PPI network was based on three major parameters of DC, BC and CC which were calculated by the CytoNCA plug-in for Cytoscape 3. According to the related potential active components, we constructed the herb-compound-target network based on 43 key targets (Figure 3E). In this network, the top seven compounds were ecdysterone, which holds relevancy to 14 key targets; bassianin, which held relevancy to 13 key targets; cholesteryl ferulate, which was related to 12 key targets; beauvericin, related to 11 key targets; hyrcanoside, ergotamine and lupeol acetate were associated with jpurnal key targets.

Продолжить чтение 2 Information on 43 Hub TargetsFigure 3 Prediction results of network pharmacology journal of medicine american NTF on ischemic stroke and CIRI. Green nodes represent the herbs of NTF, orange nodes represent the central compounds of NTF, and blue nodes represent the other active compounds of NTF. The node color changes from yellow to red reflect the degree centrality changes from low to high.

The potential therapeutic target network of NTF is presented journal of medicine american Figure 4. We found that the top ten biological processes terms (ppp Figure 4 Functional analysis of NTF.

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Comments:

28.07.2020 in 01:30 Любовь:
Ох уж эти славянки!

29.07.2020 in 20:34 Аристарх:
знаю что вроде прикольный

01.08.2020 in 08:16 lecomphyd:
Это условность